C3 抗体 (FITC)
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- 抗原 See all C3 抗体
- C3 (Complement Component 3 (C3))
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适用
- 豚鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This C3 antibody is conjugated to FITC
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunodiffusion (ID), Dot Blot (DB)
- 特异性
- In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of guinea pig serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of guinea pig plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
- 产品特性
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Absorption / Emission: 492 nm / 515 nm.
Molar Ratio: Fluorochrome/IgG ~1.6 - 纯化方法
- Hyperimmune antisera with strong precipitating activity are selected for Fractionation and purification of the IgG fraction containing the bulk of the defined antibody specificity.
- 免疫原
- C3c is isolated and purified from pooled normal guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product C3 Primary Antibody
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anti-Complement Component 3 (C3) (AA 1000-1326) antibody
C3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 1551-1663) antibodyC3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (fro), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 1534-1644) antibodyC3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 5F9 unconjugated
anti-Complement Component 3 (C3) (C-Term) antibodyC3 适用: 人 WB, IP, IF, ICC, IHC (p), ISub 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 23-300) antibodyC3 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 965-1303) antibodyC3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 1534-1645) antibodyC3 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5F9 unconjugated
anti-Complement Component 3 (C3) antibodyC3 适用: 小鼠 WB, IP, FACS, IF, IA 宿主: 大鼠 Monoclonal 11H9 unconjugated
anti-Complement Component 3 (C3) (AA 966-1313) antibodyC3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 1353-1382) antibodyC3 适用: 人 WB 宿主: 小鼠 Monoclonal 567CT11-2-3 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore by adding 1 mL of sterile distilled water
- 浓度
- 10,0 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.
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- 抗原
- C3 (Complement Component 3 (C3))
- 别名
- Complement C3 (C3 产品)
- 别名
- AHUS5 antibody, ARMD9 antibody, ASP antibody, C3a antibody, C3b antibody, CPAMD1 antibody, AI255234 antibody, HSE-MSF antibody, Plp antibody, C3 antibody, C3-1 antibody, sb:cb26 antibody, si:dkey-76b14.4 antibody, wu:fi34b03 antibody, wu:fj86e11 antibody, C3d antibody, asp antibody, cpamd1 antibody, c3 antibody, complement C3 antibody, complement component 3 antibody, complement component c3a, duplicate 1 antibody, complement component 3 L homeolog antibody, complement component C3 antibody, complement C3-like antibody, C3 antibody, c3a.1 antibody, c3.L antibody, LOC443475 antibody, c3 antibody, c3-1 antibody, LOC100060539 antibody
- 背景
- C3 is the most abundant complement protein in guinea pig serum. Its biological function strongly resembles that of C3 in man an other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response. The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently the antiserum reacts with both native and activated C3. It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain.Synonyms: CPAMD1, Complement component 3
- 基因ID
- 24232
- NCBI登录号
- NP_058690
- UniProt
- P01026
- 途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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