Biotin-conjugated IgG fraction of polyclonal sheep antiserum to pyruvate kinase from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
产品特性
Molar Ratio: Biotin/IgG ~9.1
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
Pyruvate kinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedur