Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB), Enzyme Immunoassay (EIA)
特异性
IgG fraction of polyclonal sheep antiserum to phosphorylase b from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
Phosphorylase b isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.