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PGK1 抗体

Cited in 5+ publications. This anti-PGK1 antibody is a 兔 多克隆 antibody detecting PGK1 in WB, IF, IP, EIA, DB, ID 和 RIA. Suitable for Saccharomyces cerevisiae.
产品编号 ABIN568371
发货至: 中国
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Quick Overview for PGK1 抗体 (ABIN568371)

抗原

See all PGK1 抗体
PGK1 (Phosphoglycerate Kinase 1 (PGK1))

适用

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Saccharomyces cerevisiae

宿主

  • 88
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克隆类型

  • 60
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多克隆

标记

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This PGK1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
  • 特异性

    3-Phosphoglyceric Phosphokinase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    纯化方法

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    免疫原

    3-Phosphoglyceric Phosphokinase isolated and purified from baker's yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    亚型

    IgG
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 溶解方式

    Restore by adding 1.0 mL of sterile distilled water

    浓度

    10.0 mg/mL

    缓冲液

    PBS, pH 7.2 without preservatives and foreign proteins

    储存液

    Without preservative

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Sienski, Narayan, Bonner, Kory, Boland, Arczewska, Ralvenius, Akay, Lockshin, He, Milo, Graziosi, Baru, Lewis, Kellis, Sabatini, Tsai, Lindquist: "APOE4 disrupts intracellular lipid homeostasis in human iPSC-derived glia." in: Science translational medicine, Vol. 13, Issue 583, (2021) (PubMed).

    Narayan, Sienski, Bonner, Lin, Seo, Baru, Haque, Milo, Akay, Graziosi, Freyzon, Landgraf, Hesse, Valastyan, Barrasa, Tsai, Lindquist: "PICALM Rescues Endocytic Defects Caused by the Alzheimer's Disease Risk Factor APOE4." in: Cell reports, Vol. 33, Issue 1, pp. 108224, (2021) (PubMed).

    Kayatekin, Amasino, Gaglia, Flannick, Bonner, Fanning, Narayan, Barrasa, Pincus, Landgraf, Nelson, Hesse, Costanzo, Myers, Boone, Florez, Lindquist: "Translocon Declogger Ste24 Protects against IAPP Oligomer-Induced Proteotoxicity." in: Cell, Vol. 173, Issue 1, pp. 62-73.e9, (2019) (PubMed).

    Fanning, Haque, Imberdis, Baru, Barrasa, Nuber, Termine, Ramalingam, Ho, Noble, Sandoe, Lou, Landgraf, Freyzon, Newby, Soldner, Terry-Kantor, Kim, Hofbauer, Becuwe, Jaenisch, Pincus, Clish, Walther et al.: "Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. ..." in: Molecular cell, Vol. 73, Issue 5, pp. 1001-1014.e8, (2019) (PubMed).

    Newby, Kiriakov, Hallacli, Kayatekin, Tsvetkov, Mancuso, Bonner, Hesse, Chakrabortee, Manogaran, Liebman, Lindquist, Khalil: "A Genetic Tool to Track Protein Aggregates and Control Prion Inheritance." in: Cell, Vol. 171, Issue 4, pp. 966-979.e18, (2017) (PubMed).

  • 抗原

    PGK1 (Phosphoglycerate Kinase 1 (PGK1))

    别名

    PGK1

    背景

    Synonyms: Phosphoglycerate kinase, YCR012W, YCR12W

    基因ID

    850370

    NCBI登录号

    NP_009938

    UniProt

    P00560

    途径

    Cellular Glucan Metabolic Process
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