Pepsin 抗体
Quick Overview for Pepsin 抗体 (ABIN568351)
抗原
See all Pepsin 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Pepsin from Porcine stomach mucosa. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
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纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
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免疫原
- Pepsin isolated and purified from Porcine stomach mucosa. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Pepsin antibody
适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pepsin antibody适用: 人, Pig, Hamster, 绵羊, 大鼠, 兔, 小鼠, 猴, 马, 豚鼠, 山羊, 驴, 犬, Cow, 小鸡, Cat, Avian WB 宿主: 山羊 Polyclonal unconjugated
anti-Pepsin (AA 352-381), (C-Term) antibody适用: 人 WB, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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溶解方式
- Restore by adding 1.0 mL sterile distilled water
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浓度
- 10.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Pepsin
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背景
- Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.Synonyms: PGA, PGA3, PGA4, PGA5, Pepsin A, Pepsinogen A, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I
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基因ID
- 396892
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NCBI登录号
- NP_999038
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UniProt
- P00791
抗原
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