Pepsin 抗体
The 兔 多克隆 anti-Pepsin antibody is suitable to detect Pepsin in samples from Pig. It has been validated for WB, ELISA, DB 和 IFA.
产品编号 ABIN459251
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Pepsin 抗体 (ABIN459251)
抗原
See all Pepsin 抗体
Pepsin
适用
Pig
宿主
兔
克隆类型
多克隆
标记
This Pepsin antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA, Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)
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原理
- Rabbit anti Porcine Pepsin
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特异性
- IgG fraction of polyclonal Rabbit antiSerum to pepsin from porcine stomach mucosa
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交叉反应 （详细）
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
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产品特性
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Pepsin isolated and purified from porcine stomach mucosa. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Pepsin antibody
适用: 人, Pig, Hamster, 绵羊, 大鼠, 兔, 小鼠, 猴, 马, 豚鼠, 山羊, 驴, 犬, Cow, 小鸡, Cat, Avian WB 宿主: 山羊 Polyclonal unconjugated
anti-Pepsin (AA 352-381), (C-Term) antibody适用: 人 WB, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Pepsin
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UniProt
- P00791
抗原
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