Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)
特异性
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
D-Lactate dehydrogenase, isolated and purified from Lactobacillus leichmannii. Freund’s complete adjuvant is used in the first step of the immunization procedure.