The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
D-Lactate dehydrogenase, isolated and purified from Lactobacillus leichmannii. Freund’s complete adjuvant is used in the first step of the immunization procedure.