Hexokinase 1 抗体 (Biotin)
Quick Overview for Hexokinase 1 抗体 (Biotin) (ABIN568253)
抗原
See all Hexokinase 1 (HK1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
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产品特性
- Molar Ratio: Biotin/IgG = ~5.4
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纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
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免疫原
- Hexokinase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 10.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins.
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Hexokinase 1 (HK1)
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别名
- Hexokinase-1
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背景
- Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia. In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase. Present with 40800 Molecules/cell in log phase SD medium.Synonyms: HKA, HXK1, Hexokinase PI, Hexokinase-A
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基因ID
- 850614
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NCBI登录号
- NP_116711
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UniProt
- P04806
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途径
- Carbohydrate Homeostasis, Warburg Effect
抗原
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