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Hexokinase 1 抗体 (Biotin)

This anti-Hexokinase 1 antibody is a 兔 多克隆 antibody detecting Hexokinase 1 in WB, IP, IF, EIA, RIA, ID 和 DB. Suitable for Saccharomyces cerevisiae.
产品编号 ABIN568253
发货至: 中国

Quick Overview for Hexokinase 1 抗体 (Biotin) (ABIN568253)

抗原

See all Hexokinase 1 (HK1) 抗体
Hexokinase 1 (HK1)

适用

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Saccharomyces cerevisiae

宿主

  • 116
  • 17

克隆类型

  • 107
  • 26
多克隆

标记

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This Hexokinase 1 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID), Dot Blot (DB)
  • 特异性

    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.

    产品特性

    Molar Ratio: Biotin/IgG = ~5.4

    纯化方法

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

    免疫原

    Hexokinase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    亚型

    IgG
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    10.0 mg/mL

    缓冲液

    PBS, pH 7.2 without preservatives and foreign proteins.

    储存液

    Without preservative

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • 抗原

    Hexokinase 1 (HK1)

    别名

    Hexokinase-1

    背景

    Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia. In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase. Present with 40800 Molecules/cell in log phase SD medium.Synonyms: HKA, HXK1, Hexokinase PI, Hexokinase-A

    基因ID

    850614

    NCBI登录号

    NP_116711

    UniProt

    P04806

    途径

    Carbohydrate Homeostasis, Warburg Effect
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