The antibody recognizes Glyoxalase from Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
纯化方法
Solid Phase Affinity Chromatography
免疫原
Glyoxalase isolated and purified from Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
溶解方式
Restore by adding 0.5 mL of sterile distilled water
浓度
2.0 mg/mL
缓冲液
PBS, pH 7.2 stabilized with Dextran, without preservatives and foreign proteins
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
抗原
GLO1
(Glyoxalase I (GLO1))
别名
Glyoxalase I / GLO1
背景
The enzyme encoded by GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. GLO is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.Synonyms: Aldoketomutase, Glx I, Ketone-aldehyde mutase, Methylglyoxalase, S-D-lactoylglutathione methylglyoxal lyase