The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
产品特性
Molar Ratio: ~5.4
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
免疫原
Adenosine Deaminase isolated and purified from Calf Intestine. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
溶解方式
Restore by adding 1.0 mL of sterile distilled water.
浓度
10.0 mg/mL
缓冲液
PBS, pH 7.2 without preservatives and foreign proteins.
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
抗原
ADA
(Adenosine Deaminase (ADA))
背景
ADA (Adenosine deaminase) is an enzyme involved in the purine catabolic pathway. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Defects in ADA are a cause of autosomal recessive severe combined immuno-deficiency (SCID).Synonyms: Adenosine aminohydrolase