Recombinant Flavivirus Group Antigen 抗体
Cited in 3+ publications.
The 小鼠 单克隆 anti-Flavivirus Group Antigen antibody (Clone D1-4G2-4-15 (4G2)) (ABIN5668106) specifically detects Flavivirus Group Antigen in ELISA, FACS, IF, IHC (p) 和 WB.
The antibody is reactive with Flavivirus, Dengue Virus, Virus, West Nile Virus (WNV) 和 Yellow Fever Virus samples.
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(3 references)
产品编号 ABIN5668106
发货至:
中国
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Quick Overview for Recombinant Flavivirus Group Antigen 抗体 (ABIN5668106)
抗原
Flavivirus Group Antigen
抗体类型
Recombinant Antibody
适用
Flavivirus, Dengue Virus, Virus, West Nile Virus (WNV), Yellow Fever Virus
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
克隆位点
D1-4G2-4-15 (4G2)
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原理
- Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)], Mouse IgG2a, kappa
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特异性
- Recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
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交叉反应
- Flavivirus, Virus, West Nile Virus (WNV), Yellow Fever Virus
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- Dengue Virus type 2 antigens.
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亚型
- IgG2a kappa
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anti-Flavivirus Group Antigen antibody
适用: Flavivirus, Dengue Virus, Virus, West Nile Virus (WNV), Yellow Fever Virus ELISA, FACS, IF, IHC (p), WB 宿主: 兔 Chimeric D1-4G2-4-15 (4G2) unconjugated Recombinant Antibody
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应用备注
- This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis, anti-Yellow Fever Virus or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used Ab00230-2.0 at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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: "Storage-Dependent Generation of Potent Anti-ZIKV Activity in Human Breast Milk." in: Viruses, Vol. 11, Issue 7, (2020) (PubMed).
: "Semen inhibits Zika virus infection of cells and tissues from the anogenital region. ..." in: Nature communications, Vol. 9, Issue 1, pp. 2207, (2018) (PubMed).
: "A Human Bi-specific Antibody against Zika Virus with High Therapeutic Potential. ..." in: Cell, Vol. 171, Issue 1, pp. 229-241.e15, (2017) (PubMed).
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: "Storage-Dependent Generation of Potent Anti-ZIKV Activity in Human Breast Milk." in: Viruses, Vol. 11, Issue 7, (2020) (PubMed).
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- Flavivirus Group Antigen
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物质类
- Viral Protein
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背景
- Protein E, Envelope Protein
抗原
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