Recombinant Flavivirus Group Antigen 抗体
适用: Dengue Virus, Flavivirus, Virus, West Nile Virus (WNV), Yellow Fever Virus
WB, ELISA, FACS, IF, IHC (p)
宿主: 兔
Chimeric
D1-4G2-4-15 (4G2)
unconjugated
Recombinant Antibody
1 image
(3 references)-
- 抗原
- Flavivirus Group Antigen
- 抗体类型
- Recombinant Antibody
- 适用
- Dengue Virus, Flavivirus, Virus, West Nile Virus (WNV), Yellow Fever Virus
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
- 产品特性
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OriginalSpeciesName: Mouse
OriginalFormat: IgG2a
- 纯化方法
- Purified antibody.
- 免疫原
- Dengue Virus type 2 antigens.
- 克隆位点
- D1-4G2-4-15 (4G2)
- 亚型
- IgG kappa
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anti-Flavivirus Group Antigen antibody
适用: Dengue Virus, Flavivirus, Virus, West Nile Virus (WNV), Yellow Fever Virus WB, ELISA, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal D1-4G2-4-15 (4G2) unconjugated Recombinant Antibody
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- 应用备注
- This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis, anti-Yellow Fever Virus or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used Ab00230-2.0 at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection.
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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: "Role of microglia in the dissemination of Zika virus from mother to fetal brain." in: PLoS neglected tropical diseases, Vol. 14, Issue 7, pp. e0008413, (2020) (PubMed).
: "Obstetric Ultrasonography to Detect Fetal Abnormalities in a Mouse Model for Zika Virus Infection." in: Viruses, Vol. 12, Issue 1, (2020) (PubMed).
: "Intrauterine Zika virus infection of pregnant immunocompetent mice models transplacental transmission and adverse perinatal outcomes." in: Nature communications, Vol. 8, pp. 14575, (2018) (PubMed).
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: "Role of microglia in the dissemination of Zika virus from mother to fetal brain." in: PLoS neglected tropical diseases, Vol. 14, Issue 7, pp. e0008413, (2020) (PubMed).
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- 抗原
- Flavivirus Group Antigen
- 物质类
- Viral Protein
- 背景
- Protein E, Envelope Protein
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