Recombinant EGFR (Matuzumab Biosimilar) 抗体 (Extracellular Domain)
This anti-EGFR (Matuzumab Biosimilar) antibody is a Human 单克隆 antibody detecting EGFR (Matuzumab Biosimilar) in ELISA, Anta, BR, FACS, IF 和 WB. Suitable for 人.
Quick Overview for Recombinant EGFR (Matuzumab Biosimilar) 抗体 (Extracellular Domain) (ABIN5668060)
抗原
EGFR (Matuzumab Biosimilar)
抗体类型
Recombinant Antibody
适用
人
宿主
Human
克隆类型
单克隆
标记
This EGFR (Matuzumab Biosimilar) antibody is un-conjugated
应用范围
ELISA, Antagonist (Anta), Blocking Reagent (BR), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
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抗原表位
- Extracellular Domain
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原理
- Anti-EGFR [Matuzumab], Human IgG1, kappa
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特异性
- This antibody binds the extracellular domain III of the human EGFR and does not cross react with murine EGFR. It was also reported that residues S460/G461 in EGFR domain III are essential components of the epitope. This antibody binds at a site distinct from the EGF binding site, preventing conformational re-arrangement required for dimerization, a process essential for high-affinitiy ligand binding (Schmiedel, 2008). This antibody can only recognize the active form of EGFR. The antibody binds to both glyco- and aglyco receptor forms. EGFR is a transmembrane mitogenic glycoprotein with a cytoplasmically facing tyrosine kinase site which is activated by binding of the epidermal growth factor in the external domain. It also binds transforming growth factor alpha (TGFa). EGFR a member of the epidermal growth factor family and is closely related to receptor tyrosine kinases. This receptor tyrosine kinase binds ligands of the EGF family and activates signallying cascades to convert extracellular cues into appropriate cellular responses. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer, anal cancers and glioblastoma multiforme as well as inflammatory diseases.
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产品特性
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Original Species of Ab: Human
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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纯度
- > 98 % as determined by SDS-PAGE
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内毒素水平
- Endotoxin is < 1.0 EU/mg as determined by the LAL method
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免疫原
- The parental mouse antibody was generated by immunizing BALB/c mice intraperitoneally with A431 cells in phosphate buffered saline (PBS). Later on the humanzied version of the antibody was created by grafting CDRs of the murine antibody onto human constant regions.
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亚型
- IgG1 kappa
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anti-EGFR (Matuzumab Biosimilar) (Extracellular Domain) antibody
适用: 人 ELISA, Anta, BR, FACS, IF, WB 宿主: 兔 Chimeric unconjugated Recombinant Antibody
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应用备注
- This antibody is derived from a mouse parental clone mAb 425 which has demonstrated anti-tumor activity against solid tumors in phase I clinical trials. The binding characterization of this antibody to human EGFR was done using ELISA. This antibody was also found to bind human EGFR with a similar avidity as the parental mouse antibody 425 (PMID: 1798701). The parental mouse antibody 425 in combination with C225 (Cetuximab) reduced growth and survival of EGFR overexpressing MDA-MB-468 breast cancer cells more effectively than either antibody alone. The combination was also reported to effectively inhibited AKT and MAPK phosphorylation in MDA-MB-468 cell (PMID: 18424917). This antibody was used in a phase 1 study to investigate the safety and tolerability and to explore the pharmacokinetic and pharmacodynamic profile in patients with solid tumors that express EGFR (PMID: 14701780). This antibody has been used in various phase I studies alone or in combination with other therapeutic agents to treat patients with various type of cancers (PMID: 16533873, 16622465, 19238629). A study reported that the antitumor effects of matuzumab and cetuximab depend on inhibition of EGFR downstream signaling mediated by Akt or Erk rather than on inhibition of EGFR itself (PMID: 18033688).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- EGFR (Matuzumab Biosimilar)
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背景
- ErbB-1, ERBB1, HER1, EGF, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EMD72000, mAb425, 425, mAb 425
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UniProt
- P00533
抗原
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