ENO2/NSE 抗体 (AA 416-433)
Quick Overview for ENO2/NSE 抗体 (AA 416-433) (ABIN5646903)
抗原
See all ENO2/NSE (ENO2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 416-433
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产品特性
- This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
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纯化方法
- Purified
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纯度
- Protein G affinity chromatography
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免疫原
- Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody.
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亚型
- IgG2b
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anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibody
ENO2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 325-434) antibodyENO2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 251-433) antibodyENO2 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5D3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) antibodyENO2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibodyENO2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (C-Term) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Neuron Specific Enolase antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Neuron Specific Enolase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- NSE / Neuron Specific Enolase
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背景
- This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
抗原
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