CKB 抗体
Quick Overview for CKB 抗体 (ABIN5646868)
抗原
See all CKB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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纯度
- Protein G affinity chromatography
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免疫原
- Human CKBB protein was used as the immunogen for the Creatine kinase B type antibody.
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亚型
- IgG1 kappa
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应用备注
- Optimal dilution of the Creatine kinase B type antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen Only): 0.5-1 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Creatine kinase B type antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CKB (Creatine Kinase, Brain (CKB))
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别名
- Creatine kinase B type / CKB
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背景
- Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins
抗原
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