我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

MUTYH 抗体 (AA 436-535)

Name: MUTYH 抗体 (AA 436-535)
SKU: ABIN561883
Availability: OutOfStock

MUTYH 适用: 人 WB, ELISA, IP, RNAi 宿主: 小鼠 Monoclonal 4D10 unconjugated

MUTYH monoclonal antibody (M01), clone 4D10 Western Blot analysis of MUTYH expression in Hela S3 NE .

Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1).

Detection limit for recombinant GST tagged MUTYH is approximately 1ng/ml as a capture antibody.

ELISA

Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.

Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody.

Western Blot detection against Immunogen (36.

6 images

(7 references)

产品编号 ABIN561883

发货至: 中国

Datasheet as PDF

抗原 See all MUTYH 抗体

MUTYH (MutY Homolog (E. Coli) (MUTYH))
抗原表位
16

6

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 436-535
适用
56

39

20

4

2

2

2

2

2

2

2

2

2

2

2

2

1
人
宿主
50

3

3
小鼠
Compatible Secondaries
克隆类型
53

3
单克隆
标记
34

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This MUTYH antibody is un-conjugated
应用范围
31

18

17

13

13

13

12

7

4

3

2

2

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), RNA Interference (RNAi)

原理

Mouse monoclonal antibody raised against a partial recombinant MUTYH.

序列

KLTYQVYGLA LEGQTPVTTV PPGARWLTQE EFHTAAVSTA MKKVFRVYQG QQPGTCMGSK RSQVSSPCSR KKPRMGQQVL DNFFRSHIST DAHSLNSAAQ

交叉反应

人

产品特性

Antibody Reactive Against Recombinant Protein.

免疫原

MUTYH (AAH03178, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

克隆位点

4D10

亚型

IgG1
anti-MutY Homolog (E. Coli) (MUTYH) (pan) antibody
MUTYH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) antibody
MUTYH 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Internal Region) antibody
MUTYH 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 1-265) antibody
MUTYH 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (C-Term) antibody
MUTYH 适用: 人, 小鼠, 大鼠, 猴, Cow, Pig, 犬, 兔, 驴, 绵羊, 山羊, 豚鼠, Hamster, Cat, 马, 小鸡, Avian WB 宿主: 山羊 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 436-535) antibody
MUTYH 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C8 unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) antibody
MUTYH 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Center) antibody
MUTYH 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 341-367) antibody
MUTYH 适用: 人, 小鼠 WB, IF, FACS 宿主: 兔 Polyclonal RB17460 unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Center) antibody
MUTYH 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
缓冲液

In 1x PBS, pH 7.4

注意事项

Aliquot to avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Komine, Shimodaira, Takao, Soeda, Zhang, Takahashi, Ishioka: "Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia Coli Strain." in: Human mutation, Vol. 36, Issue 7, pp. 704-11, (2015) (PubMed).

Shinmura, Goto, Tao, Kato, Suzuki, Nakamura, Matsuda, Yin, Morita, Kono, Sugimura: "Impaired 8-hydroxyguanine repair activity of MUTYH variant p.Arg109Trp found in a Japanese patient with early-onset colorectal cancer." in: Oxidative medicine and cellular longevity, Vol. 2014, pp. 617351, (2014) (PubMed).

Agustina, Hahm, Han, Tran, Chung, Park, Park, Han: "Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation." in: BMC molecular biology, Vol. 15, pp. 17, (2014) (PubMed).

Raetz, Xie, Kundu, Brinkmeyer, Chang, David: "Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells." in: Carcinogenesis, Vol. 33, Issue 11, pp. 2301-9, (2012) (PubMed).

Hahm, Chung, Agustina, Han, Yoon, Park, Kang, Park, Na, Han: "Human MutY homolog induces apoptosis in etoposide-treated HEK293 cells." in: Oncology letters, Vol. 4, Issue 6, pp. 1203-1208, (2012) (PubMed).

Suzuki, Harashima, Kamiya: "Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine." in: DNA repair, Vol. 9, Issue 5, pp. 542-50, (2010) (PubMed).

van der Post, Kets, Ligtenberg, van Krieken, Hoogerbrugge: "Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis." in: Virchows Archiv : an international journal of pathology, Vol. 454, Issue 1, pp. 25-9, (2008) (PubMed).
抗原

MUTYH (MutY Homolog (E. Coli) (MUTYH))

别名

MUTYH (MUTYH 产品)

别名

MUTYH antibody, MGC145569 antibody, CYP2C antibody, MYH antibody, 5730495A01Rik antibody, Mutyha antibody, Mutyhalpha antibody, Mutyhb antibody, Mutyhbeta antibody, Mutyhc antibody, Myh antibody, mutY DNA glycosylase antibody, mutY homolog (E. coli) antibody, A/G-specific adenine DNA glycosylase-like antibody, MUTYH antibody, mutyh antibody, LOC100373318 antibody, Mutyh antibody

背景

Full Gene Name: mutY homolog (E. coli)
Synonyms: MGC4416,MYH

基因ID

4595

途径

DNA Damage Repair