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MUTYH 抗体 (AA 436-535)

Name: MUTYH 抗体 (AA 436-535)
SKU: ABIN561883
Availability: OutOfStock

This anti-MUTYH antibody is a 小鼠单克隆 antibody detecting MUTYH in WB, ELISA, IP 和 RNAi. Suitable for 人. This Primary Antibody has been cited in 7+ publications.

MUTYH monoclonal antibody (M01), clone 4D10 Western Blot analysis of MUTYH expression in Hela S3 NE . (MUTYH 抗体 (AA 436-535))

Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). (MUTYH 抗体 (AA 436-535))

Detection limit for recombinant GST tagged MUTYH is approximately 1ng/ml as a capture antibody. (MUTYH 抗体 (AA 436-535))

ELISA

Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10. (MUTYH 抗体 (AA 436-535))

Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody. (MUTYH 抗体 (AA 436-535))

Western Blot detection against Immunogen (36. (MUTYH 抗体 (AA 436-535))

6 images

(7 references)

产品编号 ABIN561883

发货至: 中国

Quick Overview for MUTYH 抗体 (AA 436-535) (ABIN561883)

抗原

See all MUTYH 抗体

MUTYH (MutY Homolog (E. Coli) (MUTYH))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This MUTYH antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunoprecipitation (IP), RNA Interference (RNAi)

克隆位点

4D10

抗原表位
16

5

5

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 436-535

原理

Mouse monoclonal antibody raised against a partial recombinant MUTYH.

序列

KLTYQVYGLA LEGQTPVTTV PPGARWLTQE EFHTAAVSTA MKKVFRVYQG QQPGTCMGSK RSQVSSPCSR KKPRMGQQVL DNFFRSHIST DAHSLNSAAQ

交叉反应

人

产品特性

Antibody Reactive Against Recombinant Protein.

免疫原

MUTYH (AAH03178, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

亚型

IgG1
anti-MutY Homolog (E. Coli) (MUTYH) (pan) antibody
MUTYH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Internal Region) antibody
MUTYH 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 1-265) antibody
MUTYH 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (C-Term) antibody
MUTYH 适用: 人, 小鼠, 大鼠, 猴, 山羊, 绵羊, 兔, Pig, 马, Hamster, 豚鼠, 驴, 犬, Cow, 小鸡, Cat, Avian WB 宿主: 山羊 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 436-535) antibody
MUTYH 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C8 unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) antibody
MUTYH 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Center) antibody
MUTYH 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 341-367) antibody
MUTYH 适用: 人, 小鼠 WB, IF, FACS 宿主: 兔 Polyclonal RB17460 unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (Center) antibody
MUTYH 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-MutY Homolog (E. Coli) (MUTYH) (AA 352-381), (Middle Region) antibody
MUTYH 适用: 人, 小鼠 WB, IF, FACS, EIA 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
缓冲液

In 1x PBS, pH 7.4

注意事项

Aliquot to avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Komine, Shimodaira, Takao, Soeda, Zhang, Takahashi, Ishioka: "Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia Coli Strain." in: Human mutation, Vol. 36, Issue 7, pp. 704-11, (2015) (PubMed).

Shinmura, Goto, Tao, Kato, Suzuki, Nakamura, Matsuda, Yin, Morita, Kono, Sugimura: "Impaired 8-hydroxyguanine repair activity of MUTYH variant p.Arg109Trp found in a Japanese patient with early-onset colorectal cancer." in: Oxidative medicine and cellular longevity, Vol. 2014, pp. 617351, (2014) (PubMed).

Agustina, Hahm, Han, Tran, Chung, Park, Park, Han: "Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation." in: BMC molecular biology, Vol. 15, pp. 17, (2014) (PubMed).

Raetz, Xie, Kundu, Brinkmeyer, Chang, David: "Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells." in: Carcinogenesis, Vol. 33, Issue 11, pp. 2301-9, (2012) (PubMed).

Hahm, Chung, Agustina, Han, Yoon, Park, Kang, Park, Na, Han: "Human MutY homolog induces apoptosis in etoposide-treated HEK293 cells." in: Oncology letters, Vol. 4, Issue 6, pp. 1203-1208, (2012) (PubMed).

Suzuki, Harashima, Kamiya: "Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine." in: DNA repair, Vol. 9, Issue 5, pp. 542-50, (2010) (PubMed).

van der Post, Kets, Ligtenberg, van Krieken, Hoogerbrugge: "Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis." in: Virchows Archiv : an international journal of pathology, Vol. 454, Issue 1, pp. 25-9, (2008) (PubMed).
抗原

MUTYH (MutY Homolog (E. Coli) (MUTYH))

别名

MUTYH

背景

Full Gene Name: mutY homolog (E. coli)
Synonyms: MGC4416,MYH

基因ID

4595

途径

DNA Damage Repair