PDGFRA 抗体 (C-Term)
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Quick Overview for PDGFRA 抗体 (C-Term) (ABIN5611421)
抗原
See all PDGFRA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- Specific for PDGFR, alpha and does not react with PDGFR, beta. Cellular Localization Cell membrane.
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交叉反应 (详细)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Protein A Chromatography.
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免疫原
- A synthetic peptide derived from the C-terminus of the precursor form of Human PDGFR alpha.
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亚型
- IgG
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应用备注
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ELISA. Immunohistochemistry on Paraffin Sections: Use Ab at 5-10 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: Ovariancarcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer, before the addition of Glycerol to 40 %.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))
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别名
- CD140a / PDGFRA
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背景
- PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta coded by two different genes. They possess five immunoglobulin like domains that are involved in ligand binding. Two tyrosine kinase domains are separated by a kinase insert to which PI-3 kinase can bind. PDGF AA can bind autophosphorylate and activate only PDGFR, alpha while PDGF BB can bind and activate to both PDGFR, alpha and beta.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha
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基因ID
- 5156
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NCBI登录号
- NP_006197
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UniProt
- P16234
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling
抗原
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