Clusterin 抗体
(1 reference)
Quick Overview for Clusterin 抗体 (ABIN5596991)
抗原
See all Clusterin (CLU) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-101-198
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Supplier
- Rockland
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原理
- APOLIPOPROTEIN J Antibody
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交叉反应 （详细）
- Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.
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产品特性
- Synonyms: goat anti-Apolipoprotein J antibody, Clusterin, Complement-associated protein SP-40, Complement cytolysis inhibitor, CLI, NA1/NA2, Apo-J, Testosterone-repressed prostate message 2, TRPM-2, Ku70-binding protein 1, Aging-associated gene 4 protein, Clusterin beta chain, Clusterin alpha chain, Complement cytolysis inhibitor a chain, Complement cytolysis inhibitor b chain, ApoJ alpha, ApoJ beta, CLU, CLI, KUB1, AAG4
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纯化方法
- This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: apoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Clusterin (CLU) (AA 331-449) antibody
CLU 适用: 人, 大鼠, 小鼠 ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 229-446) antibodyCLU 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 227-448) antibodyCLU 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 228-449) antibodyCLU 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 226-447) antibodyCLU 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Clusterin (CLU) (C-Term) antibodyVerified CLU 适用: 人 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 23-120) antibodyCLU 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 227-447) antibodyCLU 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Clusterin (CLU) (C-Term) antibodyVerified CLU 适用: 大鼠, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 402-501) antibodyCLU 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A11 unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-apolipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:5,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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: "Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin." in: The Journal of biological chemistry, Vol. 284, Issue 43, pp. 29605-13, (2009) (PubMed).
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: "Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin." in: The Journal of biological chemistry, Vol. 284, Issue 43, pp. 29605-13, (2009) (PubMed).
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- Clusterin (CLU)
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别名
- CLU
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背景
- Background: Apolipoprotein J Antibody functions as a secreted chaperone that prevents aggregation of nonnative proteins. It prevents stress-induced aggregation of blood plasma proteins and inhibits the formations of amyloid fibrils. Apolipoprotein J does not require ATP or refold proteins by itself. It maintains partially unfolded proteins in a state for subsequent refolding by other chaperones. It is shown to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Binding to cell surface receptors it triggers internalization of chaperone-client complex and subsequent lysosomal or proteasomal degradation. It modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis while mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Anti-Apolipoprotein J Antibody is useful for researchers interested in the immune system, Ubiquitin pathways, and cardiovascular research.
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基因ID
- 1191
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NCBI登录号
- NP_001822
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UniProt
- P10909
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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