TET3 抗体 (N-Term)
Quick Overview for TET3 抗体 (N-Term) (ABIN5596980)
抗原
See all TET3 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
Supplier Product No.
- 100-401-n94
-
Supplier
- Rockland
-
原理
- TET3 Antibody
-
产品特性
- Synonyms: rabbit anti-TET3 Antibody, TET antibodies, Anti-TET Methylcytosine Dioxygenase 3, Anti-TET3 polyclonal antibody, KIAA0401 antibody, dioxygenase antibodies
-
纯化方法
- Anti-TET3 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: TET3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-3 domain containing the N-terminal 160 amino acids of the protein.
Immunogen Type: Recombinant Protein
-
-
anti-Tet Methylcytosine Dioxygenase 3 (TET3) (AA 1525-1660) antibody
TET3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 3 (TET3) (AA 72-335) antibodyTET3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 3 (TET3) (AA 1-937) antibodyTET3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 3 (TET3) (AA 1159-1329) antibodyTET3 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: Tet3 antibody has been tested by Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~180 kDa. This antibody is suitable for use by ELISA.
Western Blot Dilution: 1:5000
ELISA Dilution: 1:50,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 70 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))
-
别名
- TET3
-
背景
- Background: Anti-Tet3 is expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes. Anti-Tet3 is involved in the epigenetic chromatin reprogramming of a zygote after its fertilization, and the demethylation occurs before the first cell division in the paternal pronucleus. Tet 3 antibody cannot bind to the maternal pronucleus chromatin due to DPPA3/PGC7 chromatin presence. Tet 3 functions as the dioxygenase which synthesizes a hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet3 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet3 plays an active role in catalyzing those conversions which have been suggested as the first step for cytosine DNA de-methylation. Tet 3 also recruits O-GlcNAc transferase OGT to aid H2B GlcNAcylation by OGT. Anti-TET3 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.
-
基因ID
- 200424
-
NCBI登录号
- NP_659430
-
UniProt
- O43151
-
途径
- Warburg Effect
抗原
-
