TET3 抗体 (N-Term)
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北京 101111
Quick Overview for TET3 抗体 (N-Term) (ABIN5596980)
抗原
See all TET3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 100-401-n94
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Supplier
- Rockland
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原理
- TET3 Antibody
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产品特性
- Synonyms: rabbit anti-TET3 Antibody, TET antibodies, Anti-TET Methylcytosine Dioxygenase 3, Anti-TET3 polyclonal antibody, KIAA0401 antibody, dioxygenase antibodies
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纯化方法
- Anti-TET3 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: TET3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-3 domain containing the N-terminal 160 amino acids of the protein.
Immunogen Type: Recombinant Protein
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anti-Tet Methylcytosine Dioxygenase 3 (TET3) (AA 1525-1660) antibody
TET3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 3 (TET3) (AA 72-335) antibodyTET3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 3 (TET3) (AA 1-937) antibodyTET3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 3 (TET3) (AA 1159-1329) antibodyTET3 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Tet3 antibody has been tested by Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~180 kDa. This antibody is suitable for use by ELISA.
Western Blot Dilution: 1:5000
ELISA Dilution: 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 70 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))
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别名
- TET3
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背景
- Background: Anti-Tet3 is expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes. Anti-Tet3 is involved in the epigenetic chromatin reprogramming of a zygote after its fertilization, and the demethylation occurs before the first cell division in the paternal pronucleus. Tet 3 antibody cannot bind to the maternal pronucleus chromatin due to DPPA3/PGC7 chromatin presence. Tet 3 functions as the dioxygenase which synthesizes a hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet3 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet3 plays an active role in catalyzing those conversions which have been suggested as the first step for cytosine DNA de-methylation. Tet 3 also recruits O-GlcNAc transferase OGT to aid H2B GlcNAcylation by OGT. Anti-TET3 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.
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基因ID
- 200424
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NCBI登录号
- NP_659430
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UniProt
- O43151
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途径
- Warburg Effect
抗原
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