APOA1 抗体
(3 references)
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- 抗原 See all APOA1 抗体
- APOA1 (Apolipoprotein A-I (APOA1))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This APOA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-101-109
- Supplier
- Rockland
- 原理
- APOLIPOPROTEIN A-I Antibody
- 交叉反应 （详细）
- Typically less than 1 % cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.
- 产品特性
- Synonyms: goat anti-Apolipoprotein A-I Antibody, goat anti-APOA1 antibody, goat anti-APO-A1 antibody, goat anti-APOA-1 antibody, APOA1/APOC3 fusion gene antibody, Apolipoprotein A I precursor antibody, Apolipoprotein AI antibody, Apolipoprotein of high density lipoprotein antibody, ProapoA-I, Proapolipoprotein A-I, ApoA-I
- 纯化方法
- Goat Anti-Apolipoprotein A-I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: apoLipoprotein Type A-I was isolated from human plasma by density gradient centrifugation followed by HPLC purification, followed by repeated immunizations in goat.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product APOA1 Primary Antibody
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anti-Apolipoprotein A-I (APOA1) (AA 25-264) antibody
APOA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 122-267) antibodyAPOA1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 25-265) antibodyAPOA1 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 1-267) antibodyAPOA1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 1-267) antibodyAPOA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal S1 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 10-37) antibodyAPOA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) antibodyAPOA1 适用: 小鼠 WB, ELISA, IHC, IP 宿主: 山羊 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 51-150) antibodyAPOA1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (N-Term) antibodyAPOA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 20-267) antibodyAPOA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-apoLipoprotein antibodies have been tested by Western blot and IHC and are suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation, immunohistochemistry and for western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:1,000 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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: "Kidney proteome changes provide evidence for a dynamic metabolism and regional redistribution of plasma proteins during torpor-arousal cycles of hibernation." in: Physiological genomics, Vol. 44, Issue 14, pp. 717-27, (2012) (PubMed).
: "Cholesterol and lipoprotein dynamics in a hibernating mammal." in: PLoS ONE, Vol. 6, Issue 12, pp. e29111, (2012) (PubMed).
: "Proteomic analysis of the winter-protected phenotype of hibernating ground squirrel intestine." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 295, Issue 1, pp. R316-28, (2008) (PubMed).
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: "Kidney proteome changes provide evidence for a dynamic metabolism and regional redistribution of plasma proteins during torpor-arousal cycles of hibernation." in: Physiological genomics, Vol. 44, Issue 14, pp. 717-27, (2012) (PubMed).
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- 抗原
- APOA1 (Apolipoprotein A-I (APOA1))
- 别名
- APOA1 (APOA1 产品)
- 背景
- Background: Anti-Apolipoprotein A-I antibody recognizes the gene product of APOA1. Apolipoprotein promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease. Anti-Apolipoprotein A-I is useful for researchers interested in cardiovascular research.
- 基因ID
- 335, 4557321
- UniProt
- P02647
- 途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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