(10 references)-
- 抗原 See all APOA1 抗体
- APOA1 (Apolipoprotein A-I (APOA1))
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This APOA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other mouse serum proteins is negligible.
- 纯化方法
- This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: apoLipoprotein Type A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product APOA1 Primary Antibody
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anti-Apolipoprotein A-I (APOA1) (AA 25-264) antibody
APOA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 122-267) antibodyAPOA1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 25-265) antibodyAPOA1 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 1-267) antibodyAPOA1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 1-267) antibodyAPOA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal S1 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 10-37) antibodyAPOA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 51-150) antibodyAPOA1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) antibodyAPOA1 适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (N-Term) antibodyAPOA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 25-267) antibodyAPOA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:250 - 1:500
Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:500 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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: "Serum amyloid A impairs the antiinflammatory properties of HDL." in: The Journal of clinical investigation, Vol. 126, Issue 1, pp. 266-81, (2016) (PubMed).
: "Overexpression of Hsp27 ameliorates symptoms of Alzheimer's disease in APP/PS1 mice." in: Cell stress & chaperones, Vol. 18, Issue 6, pp. 759-71, (2014) (PubMed).
: "Abca1 deficiency affects Alzheimer's disease-like phenotype in human ApoE4 but not in ApoE3-targeted replacement mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 38, pp. 13125-36, (2012) (PubMed).
: "siRNA-induced liver ApoB knockdown lowers serum LDL-cholesterol in a mouse model with human-like serum lipids." in: Journal of lipid research, Vol. 52, Issue 6, pp. 1084-97, (2011) (PubMed).
: "Reduced body weight in male Tspan8-deficient mice." in: International journal of obesity (2005), Vol. 35, Issue 4, pp. 605-17, (2011) (PubMed).
: "An apoA-I mimetic peptide increases LCAT activity in mice through increasing HDL concentration." in: International journal of biological sciences, Vol. 5, Issue 5, pp. 489-99, (2009) (PubMed).
: "Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo." in: Journal of lipid research, Vol. 49, Issue 2, pp. 386-93, (2008) (PubMed).
: "Niacin increases HDL by reducing hepatic expression and plasma levels of cholesteryl ester transfer protein in APOE*3Leiden.CETP mice." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 11, pp. 2016-22, (2008) (PubMed).
: "Fenofibrate increases HDL-cholesterol by reducing cholesteryl ester transfer protein expression." in: Journal of lipid research, Vol. 48, Issue 8, pp. 1763-71, (2007) (PubMed).
: "Loss of polycystin-1 or polycystin-2 results in dysregulated apolipoprotein expression in murine tissues via alterations in nuclear hormone receptors." in: Human molecular genetics, Vol. 15, Issue 1, pp. 11-21, (2005) (PubMed).
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: "Serum amyloid A impairs the antiinflammatory properties of HDL." in: The Journal of clinical investigation, Vol. 126, Issue 1, pp. 266-81, (2016) (PubMed).
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- 抗原
- APOA1 (Apolipoprotein A-I (APOA1))
- 别名
- APOLIPOPROTEIN A-I (APOA1 产品)
- 别名
- Alp-1 antibody, Apoa-1 antibody, Brp-14 antibody, Ltw-1 antibody, Lvtw-1 antibody, Sep-1 antibody, Sep-2 antibody, Sep2 antibody, apoA-I antibody, apoa antibody, apoa1 antibody, cb49 antibody, wu:fb33f01 antibody, zgc:103718 antibody, MGC64335 antibody, MGC89745 antibody, Apo-AI antibody, ApoA-I antibody, APOA1 antibody, LOC100136573 antibody, apoa-i antibody, ApoAI antibody, apolipoprotein A1 antibody, apolipoprotein A-I antibody, apolipoprotein A-Ia antibody, apolipoprotein A-I S homeolog antibody, apolipoprotein A4 antibody, APOA1 antibody, Apoa1 antibody, apoa1a antibody, apoa1.S antibody, apoa1 antibody, LOC100136573 antibody, apoa-i antibody, apoa4 antibody
- 背景
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Synonyms: APOA1 antibody, APOA1/APOC3 fusion gene antibody, Apolipoprotein A I precursor antibody, Apolipoprotein AI antibody, Apolipoprotein of high density lipoprotein antibody
Background: Anti Apolipoprotein A-I antibody recognizes the gene product of APOA1. Apolipoprotein promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease. This antibody is suitable for cardiovascular research.
Gene Name: APOA1
- 基因ID
- 11806
- UniProt
- Q00623
- 途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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