Phosphotyrosine 抗体 (Biotin)
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北京 101111
Quick Overview for Phosphotyrosine 抗体 (Biotin) (ABIN5596786)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-406-262
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Supplier
- Rockland
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原理
- Phosphotyrosine Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.
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产品特性
- Synonyms: PhosphoTyrosine (pY)reactive antibody, PhosphoTyrosine (pY) polyclonal antibody, PhosphoTyrosine antibody produced in rabbit
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纯化方法
- Anti-PhosphoTyrosine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated tyrosine peptide coupled to agarose followed by conjugation to biotin and subsequent purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-PhosphoTyrosine was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.
Immunogen Type: Other
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亚型
- IgG
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anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibody (PerCP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PerCP
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 594
anti-Phosphotyrosine antibody (Biotin)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Biotin
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC
anti-Phosphotyrosine antibody (APC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 APC
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PE
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 390
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应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-PhosphoTyrosine antibody is tested for use in ELISA, immunohistochemistry-P, IP, and western blotting assays. The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA and flow cytometry.
Western Blot Dilution: 1:1,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-pY at -20° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Phosphotyrosine
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物质类
- Amino Acid
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背景
- Background: Anti-PhosphoTyrosine detects phosphorylated Tyrosines. Tyrosine is one of the 22 amino acids that are used by cells to synthesize proteins. Tyrosine has a phenol group. It functions as a receiver of phosphate groups that are transferred by way of protein kinases. Phosphorylation of the hydroxyl group changes the activity of the target protein. Anti-PhosphoTyrosine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.
抗原
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