STIM1 抗体 (C-Term)
Quick Overview for STIM1 抗体 (C-Term) (ABIN5596753)
抗原
See all STIM1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-j48
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Supplier
- Rockland
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原理
- STIM1 Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with rat, human, bovine, and mouse based on 100 % sequence homology.
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产品特性
- Synonyms: rabbit anti-STIM1 antibody, Stromal interaction molecule 1, STIM-1, STIM 1, GOK
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纯化方法
- Anti-Stim I was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Stim 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human Stim 1.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: Anti-Stim I antibody is tested for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1: 10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.03 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- STIM1 (Stromal Interaction Molecule 1 (STIM1))
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别名
- STIM1
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背景
- Background: Stim I antibody helps regulate calcium influx after the depletion of intracellular calcium stores. It functions as a calcium sensor in the endoplasmic reticulum via its EF-hand domain. After depletion of Ca+2, it is translocated from the endoplasmic reticulum to the plasma membrane so the calcium release-activated channel subunit is activated. Anti-Stim I antibody is ideal for investigators interested in Metabolism and Signal Transduction research.
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基因ID
- 6786
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NCBI登录号
- NP_003147
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UniProt
- Q13586
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途径
- TCR Signaling, BCR Signaling
抗原
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