STIM1 抗体 (Extracellular, Lumenal Loop, N-Term)
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Quick Overview for STIM1 抗体 (Extracellular, Lumenal Loop, N-Term) (ABIN7886152)
抗原
See all STIM1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 56-69, Extracellular, Lumenal Loop, N-Term
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原理
- A Rabbit Polyclonal Antibody to STIM1
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,mouse,bovine,canis - identical
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产品特性
- Anti-STIM1 (extracellular) Antibody (ABIN7043781, ABIN7044026 and ABIN7044027) is a highly specific antibody directed against an epitope of the human Stromal interaction molecule 1. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize STIM1 from human, rat, and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1
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亚型
- IgG
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anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-685) antibody
STIM1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 1-211) antibodySTIM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibodySTIM1 适用: 人, 大鼠, 小鼠 WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal CDN3H4 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) antibodyKD Validated STIM1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2415 unconjugated Recombinant Antibody
anti-Stromal Interaction Molecule 1 (STIM1) (AA 45-74), (N-Term) antibodySTIM1 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-686) antibodySTIM1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-686) antibodySTIM1 适用: 人 WB, IHC, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 509-538), (C-Term) antibodySTIM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB19222 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 56-69), (Extracellular), (Lumenal Loop), (N-Term) antibody (Atto 550)STIM1 适用: 人 IHC, IF, FACS, IC, LCI 宿主: 兔 Polyclonal Atto 550
anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibodySTIM1 适用: 人, 大鼠, 小鼠 WB, IHC, IP, IHC (p), ICC, InhibAb 宿主: 小鼠 Monoclonal CDN3H4 unconjugated
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应用备注
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WB: 1:1000
FC: 5 μg per reaction
ICC: 1:50
IHC: 1:100
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7237292)
Blocking Peptide: (ABIN7237292)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- STIM1 (Stromal Interaction Molecule 1 (STIM1))
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别名
- STIM1
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背景
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Synonyms: Stromal interaction molecule 1, GOK
Description: Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+-binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 Molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.
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基因ID
- 6786
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NCBI登录号
- NM_003156
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UniProt
- Q13586
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途径
- TCR Signaling, BCR Signaling
抗原
-
