HDAC9 抗体 (N-Term)
Quick Overview for HDAC9 抗体 (N-Term) (ABIN5596729)
抗原
See all HDAC9 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 600-401-j73
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Supplier
- Rockland
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原理
- HDAC9 (N-terminus) Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with human based on 100 % sequence homology.
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产品特性
- Synonyms: rabbit anti-HDAC-9 antibody, HDAC9, HDAC 9, HD-9, HD 9, Histone deacetylase 9, Histone deacetylase-9, HD9, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC7, HDAC7B, HDRP, MITR
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纯化方法
- Anti-HDAC9 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-HDAC9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide at the N-terminal of human HDAC9 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: Anti-HDAC9 antibody is tested by Immunohistochemistry and useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~111 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1: 10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.07 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- HDAC9 (Histone Deacetylase 9 (HDAC9))
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别名
- HDAC9
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背景
- Background: HDAC9 is located in the nucleus, expressed most highly in brain, heart, muscle, and testis. It is responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). The result of deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC9 represses MEF2-dependent transcription by recruiting HDAC1 and 3. It appears to inhibit skeletal myogenesis and be a factor in heart development. By repressing JUN transcription via HDAC1 and inhibiting JUN phosphorylation by MAPK10, HDAC9 protects neurons from apoptosis. Anti-HDAC9 therefore is ideal for investigators interested in Cardiovascular or Epigenetics and Nuclear Signaling research.
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UniProt
- Q9UKV0
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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