HDAC8 抗体 (C-Term)
Quick Overview for HDAC8 抗体 (C-Term) (ABIN5596727)
抗原
See all HDAC8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-j71
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Supplier
- Rockland
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原理
- HDAC8 (C-terminus) Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with rat, mouse, human, and bovine based on 100 % sequence homology.
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产品特性
- Synonyms: rabbit anti-HDAC-8 antibody, HDAC8, HDAC 8, HD-8, HD 8, Histone deacetylase 8, Histone deacetylase-8, HD8, HDACL1, CDA07
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纯化方法
- Anti-HDAC8 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-HDAC8 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human HDAC8 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Application Note: Anti-HDAC8 antibody has been tested by ELISA and western blot and is useful in Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~41 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1: 10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- HDAC8 (Histone Deacetylase 8 (HDAC8))
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别名
- HDAC8
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背景
- Background: HDAC8 is located in the nucleus and cytoplasm, expressed weakly in most tissues with higher expression in the heart, brain, kidney, pancreas, liver, lunch, placenta, prostate, and kidney. It is responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). The result of deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Anti-HDAC8 therefore is ideal for investigators interested in Stem Cells , Cardiovascular , or Epigenetics and Nuclear Signaling research.
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UniProt
- Q9BY41
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途径
- Cellular Glucan Metabolic Process
抗原
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