HDAC5 抗体 (AA 325-375)
Quick Overview for HDAC5 抗体 (AA 325-375) (ABIN5596725)
抗原
See all HDAC5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 325-375
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Supplier Product No.
- 600-401-j69
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Supplier
- Rockland
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原理
- HDAC5 (internal) Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with human and hamster based on 100 % sequence homology.
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产品特性
- Synonyms: rabbit anti-HDAC-5 antibody, HDAC5, HDAC 5, HD 5 antibody, histone deacetylase 5 antibody, Histone deacetylase-5, HD5 antibody, NY-CO-9
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纯化方法
- Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 325-375aa of human HDAC5 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: Anti-HDAC5 antibody tested in ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~122 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1: 10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- HDAC5 (Histone Deacetylase 5 (HDAC5))
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别名
- HDAC5
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背景
- Background: HDAC5 is a ubiquitous protein responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. A tag for epigenetic repression, Anti-HDAC5 is an enzyme involved in transcriptional regulation, cell cycle progression and developmental events. HDAC5 is a class II histone deacetylase as defined by its similarities to other members of this class. HDAC5 is a major player in muscle differentiation, acting by repressing transcription of myocyte enhancer MEF2C. During differentiation it moves from the nucleus into the cytoplasm, allowing for myocyte enhancer factor expression. Anti-HDAC5 antibody is ideal for researchers interested in Stem Cell , Cardiovascular , or Epigenetics and Nuclear Signaling research.
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UniProt
- Q9UQL6
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process
抗原
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