GATA4 抗体
Quick Overview for GATA4 抗体 (ABIN5596711)
抗原
See all GATA4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-p15
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Supplier
- Rockland
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原理
- GATA4 Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93 % homology.
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产品特性
- Synonyms: mouse anti-GATA4 Antibody, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody
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纯化方法
- Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG1 kappa
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应用备注
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Application Note: Anti-GATA4 antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500-1:1000
ELISA Dilution: 1:2000-1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GATA4 (GATA Binding Protein 4 (GATA4))
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别名
- GATA4
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背景
- Background: GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
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基因ID
- 2626
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NCBI登录号
- NP_002043
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UniProt
- P43694
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途径
- Peptide Hormone Metabolism, Carbohydrate Homeostasis
抗原
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