FTH1 抗体 (HRP)
Quick Overview for FTH1 抗体 (HRP) (ABIN5596710)
抗原
See all FTH1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
 - 200-403-090-0100
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Supplier
 - Rockland
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原理
 - Ferritin Antibody Peroxidase Conjugated
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交叉反应 (详细)
 - Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].
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产品特性
 - Synonyms: rabbit anti-Ferritin Antibody HRP Conjugation, peroxidase conjugated rabbit anti-Ferritin Antibody, FTHL6, Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH
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纯化方法
 - Anti-Ferritin (Human Spleen) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Ferritin [Human Spleen]
Immunogen Type: Native Protein
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亚型
 - IgG
 
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Ferritin (Human Spleen) Peroxidase Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Ferritin [Human Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Ferritin (Human Spleen) Antibody.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
 - 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 
                                            
储存液
 - Gentamicin sulfate
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注意事项
 - This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C,-20 °C
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储存方法
 - Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
 - 12 months
 
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- FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))
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别名
 - FTH1
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背景
 - Background: Ferritin is a intracellular protein that directly stores iron and regulates its release, specifically acting as a buffer for iron in humans. Ferritin is composed of 24 subunits and comes in two types, H and L. The H subunit detoxifies iron while the L subunit deals with long term storage of iron. Depending on the concentration of ferritin in the blood, different disorders and diseases could be present in an individual. Anti-Ferritin (Human Spleen) Antibody is ideal for investigators in Cancer, Immunology, and Cardiovascular Research.
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基因ID
 - 2495
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UniProt
 - P02794
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途径
 - Transition Metal Ion Homeostasis
 
抗原
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