DNASE1 抗体 (HRP)
Quick Overview for DNASE1 抗体 (HRP) (ABIN5596707)
抗原
See all DNASE1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4361-0100
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Supplier
- Rockland
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原理
- Deoxyribonuclease I Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas].
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产品特性
- Synonyms: rabbit anti-Deoxyribonuclease I Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Deoxyribonuclease I Antibody, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I
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纯化方法
- Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Deoxyribonuclease I [Bovine Pancreas]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibody
DNASE1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 22-282) antibodyDNASE1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) antibodyDNASE1 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 1-252) antibodyDNASE1 适用: 人 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibodyDNASE1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal 2-1# unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 78-231) antibodyDNASE1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 87-121) antibodyDNASE1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB49355 unconjugated
anti-Deoxyribonuclease I (DNASE1) antibodyDNASE1 适用: 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (N-Term) antibodyDNASE1 适用: 人, 大鼠, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibodyDNASE1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 7# unconjugated
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应用备注
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Application Note: Anti-Deoxyribonuclease I Peroxidase Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Deoxyribonuclease I [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- DNASE1 (Deoxyribonuclease I (DNASE1))
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别名
- DNASE1, DNL1
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背景
- Background: Deoxyribonuclease I (DNase I) is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.
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基因ID
- 282217
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NCBI登录号
- NP_776959
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UniProt
- P00639
抗原
-
