PCK1 抗体 (HRP)
Quick Overview for PCK1 抗体 (HRP) (ABIN5596694)
抗原
See all PCK1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4363-0100
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Supplier
- Rockland
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原理
- Phospho Enol Pyruvate Carboxylase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].
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产品特性
- Synonyms: rabbit anti-Phospho Enol Pyruvate Carboxylase Antibody Peroxidase Conjugation, PE conjugated rabbit anti-PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody
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纯化方法
- Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Phospho-enol-pyruvate Carboxylase [Maize Leaves]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 592-622), (C-Term) antibody
PCK1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF 宿主: 兔 Polyclonal RB03646 unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibodyPCK1 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibodyPCK1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibodyPCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 312-563) antibodyPCK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibodyPCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 330-379) antibodyPCK1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 猴, 小鸡, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibodyPCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 311-558) antibodyPCK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibodyPCK1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Phosphoenolpyruvate Carboxylase Peroxidase conjugated antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Phospho-enol-Pyruvate Carboxylase [Maize] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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别名
- Phospho Enol Pyruvate Carboxylase
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背景
- Background: Phospho Enol Pyruvate Carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form a four-carbon compound oxaloacetate. This reaction is used for carbon fixation in CAM and C4 plants where it plays a key role in photosynthesis. The enzyme is also found in some bacteria, but not in animals or fungi.
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基因ID
- 542372
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UniProt
- B8XPZ2
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途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
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