Papain 抗体 (HRP)
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- 抗原
- Papain
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适用
- Papaya
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
- Supplier Product No.
- 200-1374-0100
- Supplier
- Rockland
- 原理
- Papain Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].
- 产品特性
- Synonyms: goat anti-Papain Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody
- 纯化方法
- Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Papain (AA 134-345) antibody
适用: Papaya ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Papain antibody (Biotin)适用: Papaya ELISA, WB, IHC, IP 宿主: 山羊 Polyclonal Biotin
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- 应用备注
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Immunohistochemistry Dilution: 1:200- 1:1,000
Application Note: Anti-Papain peroxidase antibody has been tested by ELISA, dot blot, and western blot. This product is assayed against 1.0 μg of Papain [Carica papaya] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:500- 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Papain
- 背景
- Background: Papain Antibody detects Papain protein from papaya. Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans. Anti-Papain Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
- UniProt
- P00784
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