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- 抗原
- Papain
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适用
- Papaya
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 交叉反应 （详细）
- Cross reactivity against Papain from other sources may occur but has not been specifically determined.
- 纯度
- Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.
Immunogen Type: Native Protein - 亚型
- IgG
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anti-Papain (AA 134-345) antibody
适用: Papaya ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Papain antibody (HRP)适用: Papaya ELISA, WB, IHC 宿主: 山羊 Polyclonal HRP
anti-Papain antibody (Biotin)适用: Papaya ELISA, WB, IP 宿主: 山羊 Polyclonal Biotin
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- 应用备注
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Anti-Papain antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:10.000 - 1:50.000
Immunohistochemistry Dilution: 1:500 - 1:3.000
Western Blot Dilution: 1:1.000 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- Papain
- 别名
- PPI antibody, Papain antibody, papain-like antibody, LOC110813108 antibody
- 背景
- Papain Antibody detects Papain protein from papaya. Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans. Anti-Papain Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research. Synonyms: Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody
- UniProt
- P00784
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