PLG 抗体 (HRP)
Quick Overview for PLG 抗体 (HRP) (ABIN5596692)
抗原
See all PLG 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-103-208-0100
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Supplier
- Rockland
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原理
- Plasminogen Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma].
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产品特性
- Synonyms: goat anti-Plasminogen Antibody, HRP Conjugated goat anti-Plasminogen Antibody, Angiostatin antibody, Microplasmin antibody, Plasmin antibody, Plasmin heavy chain A antibody, Plasmin light chain B antibody, PLG antibody
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纯化方法
- Anti-Plasminogen is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Plasminogen [Human Plasma]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Plasminogen (PLG) antibody
PLG 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen (PLG) (AA 191-433) antibodyPLG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen (PLG) (AA 1-810) antibodyPLG 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen (PLG) (AA 21-120) antibodyPLG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A10 unconjugated
anti-Plasminogen (PLG) (AA 274-560) antibodyPLG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen (PLG) antibodyPLG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen (PLG) (AA 1-810) antibodyPLG 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Plasminogen (PLG) (AA 20-300) antibodyPLG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen (PLG) (AA 582-812) antibodyPLG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plasminogen (PLG) (AA 636-665), (C-Term) antibodyPLG 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18479 unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:250 - 1:1,000
Application Note: Anti-Plasminogen has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Plasminogen [Human Plasma] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PLG (Plasminogen (PLG))
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别名
- PLG
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背景
- Background: Plasmin is released as a zymogen called plasminogen (PLG) from the liver into the systemic circulation. Two major glycoforms of plasminogen are present in humans. Type II plasminogen is preferentially recruited to the cell surface over the type I glycoform. Conversely, type I plasminogen appears more readily recruited to blood clots. In circulation, plasminogen adopts a closed, activation resistant conformation. Upon binding to clots, or to the cell surface, plasminogen adopts an open form that can be converted into active plasmin by a variety of enzymes, including tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Fibrin is a cofactor for plasminogen activation by tissue plasminogen activator. Urokinase plasminogen activator receptor (uPAR) is a cofactor for plasminogen activation by urokinase plasminogen activator. The conversion of plasminogen to plasmin involves the cleavage of the peptide bond between Arg-561 and Val-562. Deficiency in plasmin may lead to thrombosis, as clots are not degraded adequately. Plasminogen deficiency in mice leads to defective liver repair, defective wound healing, reproductive abnormalities. In humans, a rare disorder called plasminogen deficiency type I is caused by mutations of the PLG gene and is often manifested by ligneous conjunctivitis.
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基因ID
- 5340
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UniProt
- P00747
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途径
- Complement System, Lipid Metabolism
抗原
-
