Recombinant TET1 抗体
Quick Overview for Recombinant TET1 抗体 (ABIN5563986)
抗原
See all TET1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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品牌
- AbFlex®
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产品特性
- AbFlex™ TET1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TET1(Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via TET proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. TET1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters.
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纯化方法
- Ni-NTA
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亚型
- IgG2a
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anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 2038-2136) antibody
TET1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F4 unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 1700-1800) antibodyTET1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 1845-1879), (C-Term) antibodyTET1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52496 unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (C-Term) antibodyTET1 适用: 人, 小鼠 WB, IHC, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (C-Term) antibodyTET1 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- ChIP-Seq: 4 μL per ChIP Western Blot: 1-2 μg/mL
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说明
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AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, and 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))
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别名
- TET1
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分子量
- 215 kDa
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途径
- Stem Cell Maintenance, Warburg Effect
抗原
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