Recombinant KDM4A 抗体
Quick Overview for Recombinant KDM4A 抗体 (ABIN5563977)
抗原
See all KDM4A 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
-
-
品牌
- AbFlex®
-
特异性
- Mouse
-
产品特性
- AbFlex™ JMJD2A antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.
-
纯化方法
- Protein A Chromatography
-
亚型
- IgG2a
-
-
anti-Lysine (K)-Specific Demethylase 4A (KDM4A) (AA 141-310) antibody
KDM4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysine (K)-Specific Demethylase 4A (KDM4A) (AA 932-1057) antibodyKDM4A 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6E10G4 unconjugated
anti-Lysine (K)-Specific Demethylase 4A (KDM4A) (AA 585-815) antibodyKDM4A 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Lysine (K)-Specific Demethylase 4A (KDM4A) antibodyKDM4A 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 5H1 unconjugated
anti-Lysine (K)-Specific Demethylase 4A (KDM4A) (AA 127-364) antibodyKDM4A 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysine (K)-Specific Demethylase 4A (KDM4A) antibodyKDM4A 适用: 人 DB, ICC 宿主: 小鼠 Monoclonal 3559D5a unconjugated
anti-Lysine (K)-Specific Demethylase 4A (KDM4A) (Center) antibodyKDM4A 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- WB: 1 - 2 μg/mL dilution Bead-based ELISA: 40 - 600 ng/mL
-
说明
-
AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
-
- KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))
-
别名
- JMJD2A
-
分子量
- 130 kDa
-
途径
- Warburg Effect
抗原
-
