Recombinant 5-Methylcytosine (5MeC) 抗体
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北京 101111
Quick Overview for Recombinant 5-Methylcytosine (5MeC) 抗体 (ABIN5563960)
抗原
抗体类型
宿主
克隆类型
标记
应用范围
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品牌
- AbFlex®
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特异性
- Other (Not Species Specific)
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纯化方法
- Ni-NTA
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亚型
- IgG2a
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anti-5-Methylcytosine (5MeC) antibody
5MeC WB, FACS, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-5-Methylcytosine (5MeC) antibody5MeC 适用: 人 IF, MeDIP, DB 宿主: 小鼠 Monoclonal A1 unconjugated
anti-5-Methylcytosine (5MeC) antibody (FITC)5MeC IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD FITC
anti-5-Methylcytosine (5MeC) antibody5MeC IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD unconjugated
anti-5-Methylcytosine (5MeC) antibody (Biotin)5MeC IF, ICC 宿主: 小鼠 Monoclonal 5MC-CD Biotin
anti-5-Methylcytosine (5MeC) antibody5MeC MeDIP, DB, MeDIP-Seq 宿主: 小鼠 Monoclonal A1 unconjugated
anti-5-Methylcytosine (5MeC) antibody5MeC WB, FACS, IF, MeDIP, DB, ICC, ELISA, IHC 宿主: 小鼠 Monoclonal 33D3 unconjugated
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应用备注
- Dot Blot: 1 μg / mL
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说明
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AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, and 30 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- 5-Methylcytosine (5MeC)
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别名
- 5-methylcytosine
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物质类
- Chemical
抗原
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