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Recombinant Rpb1 CTD 抗体 (pSer5)

适用: 人 WB, IF, ICC, ChIP, ChIP-seq, ELISA 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
产品编号 ABIN5563956
发货至: 中国
  • 抗原 See all Rpb1 CTD products
    Rpb1 CTD (RNA Polymerase II CTD)
    抗体类型
    Recombinant Antibody
    抗原表位
    • 14
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    pSer5
    适用
    • 36
    • 20
    • 16
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 22
    • 11
    • 3
    小鼠
    克隆类型
    • 22
    • 14
    单克隆
    标记
    • 22
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Rpb1 CTD antibody is un-conjugated
    应用范围
    • 22
    • 16
    • 13
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), ELISA
    品牌
    AbFlex®
    特异性
    Human
    产品特性
    AbFlex™ RNA pol II CTD phospho Ser5 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol IIcontains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.
    纯化方法
    Ni-NTA
    亚型
    IgG2a
  • 应用备注
    ChIP-Seq: 4 μg each ICC/IF: 1 - 2 μg / mL WB: 0.5 - 2 μg/mL Bead-based ELISA: 0.1 - 5 μg/mL
    说明

    AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
    AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

    AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • 抗原
    Rpb1 CTD (RNA Polymerase II CTD)
    别名
    RNA pol II CTD (Rpb1 CTD 产品)
    别名
    5 antibody, 8WG16 antibody, CG1554 antibody, CTD antibody, Dmel\\CG1554 antibody, H5 antibody, II antibody, L5 antibody, POL antibody, Pol II antibody, Pol II CTD antibody, Pol II Ser5P antibody, Pol II0[ser2] antibody, Pol II0[ser5] antibody, Pol II[ser2] antibody, Pol IIa antibody, Pol IIo antibody, Pol IIo[Ser2] antibody, Pol IIo[ser2] antibody, Pol IIo[ser5] antibody, Pol-IIa antibody, PolII antibody, PolIIo antibody, RNA Pol II antibody, RNA Pol II CTD antibody, RNA PolI 215 antibody, RNA PolII antibody, RNA pol II antibody, RNA pol IIo antibody, RNA polII antibody, RNAP antibody, RNAP II antibody, RNAP II LS antibody, RNAPII antibody, RNAPII0 antibody, RNApol2 antibody, RNApolII antibody, RPB1 antibody, RPII215 antibody, RpII antibody, Rpb1 antibody, Rpll215 antibody, Ubl antibody, dRPB1 antibody, dRpb1 antibody, l(1)10Ca antibody, l(1)DC912 antibody, l(1)DF912 antibody, l(1)G0040 antibody, l(1)L5 antibody, pol II antibody, polII antibody, RNA polymerase II 215kD subunit antibody, RpII215 antibody
    分子量
    240 kDa
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