Recombinant Rpb1 CTD 抗体 (pSer5)
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Quick Overview for Recombinant Rpb1 CTD 抗体 (pSer5) (ABIN5563956)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer5
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品牌
- AbFlex®
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特异性
- Human
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产品特性
- AbFlex™ RNA pol II CTD phospho Ser5 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol IIcontains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.
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纯化方法
- Ni-NTA
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亚型
- IgG2a
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anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5), (Ser5) antibody适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5), (Ser5) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer7) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3D4A12 unconjugated
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 1H4B6 unconjugated
anti-RNA Polymerase II CTD (pThr4) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, Saccharomyces cerevisiae WB, IF, ICC, ELISA, FACS 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-RNA Polymerase II CTD (pTyr1) antibody适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
anti-RNA Polymerase II CTD (pSer5), (Ser5) antibody适用: 人 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E8 unconjugated
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应用备注
- ChIP-Seq: 4 μg each ICC/IF: 1 - 2 μg / mL WB: 0.5 - 2 μg/mL Bead-based ELISA: 0.1 - 5 μg/mL
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说明
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AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- Rpb1 CTD (RNA Polymerase II CTD)
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别名
- RNA pol II CTD
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分子量
- 240 kDa
抗原
-
