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SMAD5 抗体 (pSer463, pSer465)

Name: SMAD5 抗体 (pSer463, pSer465)
SKU: ABIN5557527
Availability: OutOfStock

This anti-SMAD5 antibody is a 兔单克隆 antibody detecting SMAD5 in WB, IF (cc) 和 IHC (p). Suitable for 人, 小鼠和大鼠.

Lane 1: Mouse brain lysates, Lane 2: Rat brain lysates probed with Smad5(S463/465) (1B8) Monoclonal Antibody at 1:1000. (SMAD5 抗体 (pSer463, pSer465))

HepG2 cells were stained with Smad5(S463/465) (1B8) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (SMAD5 抗体 (pSer463, pSer465))

IF (cc)

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with Smad5(S463/465) (1B8) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (SMAD5 抗体 (pSer463, pSer465))

IHC (p)

3 images

产品编号 ABIN5557527

发货至: 中国

Quick Overview for SMAD5 抗体 (pSer463, pSer465) (ABIN5557527)

抗原

See all SMAD5 抗体

SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

单克隆

标记

This SMAD5 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

1B8

Discover our top product SMAD5 Primary Antibody

抗原表位
28

28

15

13

8

7

5

3

3

2

2

1

1

1

1

1

1

1
pSer463, pSer465

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465:IS(p-S)V(p-S).

亚型

IgG
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 210-464) antibody
SMAD5 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 140-240) antibody
SMAD5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibody
SMAD5 适用: 人, 大鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3H9 unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 1-209) antibody
SMAD5 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 105-182) antibody
SMAD5 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3A10 unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 186-465) antibody
SMAD5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 105-182) antibody
SMAD5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5E12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibody
SMAD5 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 248-267), (Middle Region) antibody
SMAD5 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 241-269) antibody
SMAD5 适用: 人 WB 宿主: 兔 Polyclonal RB40948 unconjugated

应用备注

WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

别名

Smad5

背景

Synonyms: DWFC, JV5-1, MADH5, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, SMAD family member 5, SMAD 5, Smad5, hSmad5
Background: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

基因ID

4090

UniProt

Q99717