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KRT20 抗体

This anti-KRT20 antibody is a 兔单克隆 antibody detecting KRT20 in WB, IHC (p) 和 IF (cc). Suitable for 人, 小鼠和大鼠.

Lane 1: Lovo Cell lysates, Lane 2: HCT116 Cell lysates, Lane 3: CRC lysates, probed with Cytokeratin 20 (1F10) Monoclonal Antibody at 1:1000 overnight at 4˚C. (KRT20 抗体)

CRC cells were fixed in paraformaldehyde, permeabilized with 0. (KRT20 抗体)

Paraformaldehyde-fixed, paraffin embedded Rat small intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with Cytokeratin 20 (1F10) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (KRT20 抗体)

3 images

产品编号 ABIN5557443

发货至: 中国

Datasheet as PDF

Quick Overview for KRT20 抗体 (ABIN5557443)

抗原

See all KRT20 抗体

KRT20 (Keratin 20 (KRT20))

适用

150
30
29
7
6
2
2
1
1

人, 小鼠, 大鼠

宿主

兔

克隆类型

单克隆

标记

86
17
7
5
5
5
5
5
5
5
2
2
2
2
2
2
1
1
1
1

This KRT20 antibody is un-conjugated

应用范围

128
109
51
33
24
21
18
15
13
5
4
4
3
3
2
2
1

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

克隆位点

1F10

Discover our top product KRT20 Primary Antibody

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

Recombinant human Cytokeratin 20 protein, around C-terminal 150aa.

亚型

IgG
anti-Keratin 20 (KRT20) antibody
KRT20 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Keratin 20 (KRT20) (AA 1-424) antibody
KRT20 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2G3-1C8 unconjugated

anti-Keratin 20 (KRT20) (AA 245-424) antibody
KRT20 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Keratin 20 (KRT20) (AA 196-323) antibody
KRT20 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal KRT20-1992 unconjugated

anti-Keratin 20 (KRT20) (AA 196-323) antibody
KRT20 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal KRT20-1991 unconjugated

anti-Keratin 20 (KRT20) (AA 1-266) antibody
KRT20 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4A2 unconjugated

anti-Keratin 20 (KRT20) (AA 196-323) antibody
KRT20 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal KRT20-3129R unconjugated Recombinant Antibody

anti-Keratin 20 (KRT20) (AA 196-323) antibody
KRT20 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal KRT20-3145 unconjugated

anti-Keratin 20 (KRT20) (AA 1-150) antibody
KRT20 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C7B9 unconjugated

anti-Keratin 20 (KRT20) (AA 9-424) antibody
KRT20 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

KRT20 (Keratin 20 (KRT20))

别名

Cytokeratin 20

背景

Synonyms: K20, CD20, CK20, CK-20, KRT21, Keratin, type I cytoskeletal 20, Cytokeratin-20, Keratin-20, Protein IT, KRT20
Background: Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

基因ID

54474

UniProt

P35900

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