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Cyclin B2 抗体

The 兔单克隆 anti-Cyclin B2 antibody is suitable to detect Cyclin B2 in samples from 人, 小鼠和大鼠. It has been validated for WB, IF (cc) 和 IHC (p).

Lane 1: Hela, Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody at 1:1000 overnight at 4˚C. (Cyclin B2 抗体)

PC12 cells were stained with Cyclin B2 (2F4) Monoclonal Antibody at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection. (Cyclin B2 抗体)

Paraformaldehyde-fixed, paraffin embedded mouse colon section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal serum) at 37°C for 20min, Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Cyclin B2 抗体)

Paraformaldehyde-fixed, paraffin embedded mouse testis section, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal serum) at 37°C for 20min, Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Cyclin B2 抗体)

4 images

产品编号 ABIN5557369

发货至: 中国

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Quick Overview for Cyclin B2 抗体 (ABIN5557369)

抗原

See all Cyclin B2 (CCNB2) 抗体

Cyclin B2 (CCNB2)

适用

113
51
34
12
4
4
4
4
2
2
2
2

人, 小鼠, 大鼠

宿主

兔

克隆类型

单克隆

标记

This Cyclin B2 antibody is un-conjugated

应用范围

58
50
50
24
18
14
14
13
13
4
3
2
1
1

Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

2F4

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

Recombinant human Cyclin B2 protein, around N-terminal 150aa.

亚型

IgG
anti-Cyclin B2 (CCNB2) (AA 1-100) antibody
CCNB2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (AA 58-398) antibody
CCNB2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (AA 7-163) antibody
CCNB2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) (Internal Region) antibody
CCNB2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) antibody
KD Validated CCNB2 适用: 人 WB 宿主: 兔 Monoclonal 25GB2920 unconjugated Recombinant Antibody

anti-Cyclin B2 (CCNB2) (AA 221-320) antibody
CCNB2 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B2 (CCNB2) antibody
CCNB2 适用: 人, 小鼠, 大鼠, 非洲爪蟾 ELISA, IHC, Coat 宿主: 小鼠 Monoclonal X29-2 unconjugated

anti-Cyclin B2 (CCNB2) antibody
CCNB2 适用: 人 DB 宿主: 小鼠 Monoclonal 2115C1 unconjugated

anti-Cyclin B2 (CCNB2) (pThr359) antibody
CCNB2 适用: 人 DB 宿主: 兔 Polyclonal RB42151 unconjugated

anti-Cyclin B2 (CCNB2) (pSer392) antibody
CCNB2 适用: 人 DB 宿主: 兔 Polyclonal RB42149 unconjugated

应用备注

WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

Cyclin B2 (CCNB2)

别名

Cyclin B2

背景

Synonyms: HsT17299, G2/mitotic-specific cyclin-B2, CCNB2
Background: Essential for the control of the cell cycle at the G2/M (mitosis) transition.

基因ID

9133

UniProt

O95067

途径

Cell Division Cycle, M Phase

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