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LC3B 抗体 (N-Term)

Name: LC3B 抗体 (N-Term)
SKU: ABIN1449599
Availability: OutOfStock

This anti-LC3B antibody is a 兔多克隆 antibody detecting LC3B in WB, IF, IHC (p) 和 EIA. Suitable for 人和大鼠.

Immunoblots for LC3 protein (LC3B Antibody ) are shown for extracts from untreated or rapamycin-treated (250 nM, 4 h) cultures of wild-type (CbCln3+/+) or homozygous cerebellar cells (CbCln3Äex7/8/Äex7/8). (LC3B 抗体 (N-Term))

FACS sorted LSK cells (Lineage-Sca-1+c-kit+, enriched for hematopoietic stem cells) incubated with LC3B Antibody at 1/50 dilution at 4 degree overnight. (LC3B 抗体 (N-Term))

Immunofluorescence analysis of LC3B Antibody in Rat brain before (left) and after (right) treatment with rapamycin (100 nm for 4 hr). (LC3B 抗体 (N-Term))

Formalin-Fixed, Paraffin-Embedded human testis tissue reacted with Autophagy LC3B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (LC3B 抗体 (N-Term))

IHC (p)

Wild-type (Cln3+/+) or homozygous Cln3ex7/8 (Cln3ex7/8/ex7/8) paraffin-embedded brain sections immunostained for the LC3 protein (Autophagy LC3B Antibody ). (LC3B 抗体 (N-Term))

IHC (p)

Western blot analysis of LC3B Antibody in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2 µg/lane). (LC3B 抗体 (N-Term))

Western blot analysis of LC3B Antibody in Rat brain lysate. (LC3B 抗体 (N-Term))

7 images

产品编号 ABIN1449599

发货至: 中国

Quick Overview for LC3B 抗体 (N-Term) (ABIN1449599)

抗原

See all LC3B (MAP1LC3B) 抗体

LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

适用

人, 大鼠

宿主

兔

克隆类型

多克隆

标记

This LC3B antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Discover our top product MAP1LC3B Primary Antibody

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AA 1-30, N-Term

纯化方法

Saturated Ammonium Sulfate precipitation followed by dialysis against PBS

免疫原

KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human LC3

亚型

Ig Fraction
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
KO Validated MAP1LC3B 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5H12 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 74-106) antibody
MAP1LC3B 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-30), (N-Term) antibody
MAP1LC3B 适用: 人, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody
MAP1LC3B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 人 WB, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 89-122), (cleaved) antibody
MAP1LC3B 适用: 人, 小鼠 IF, ICC 宿主: 兔 Polyclonal RB15839-RB28608 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 77-106) antibody
MAP1LC3B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Liquid

浓度

0.25 mg/mL

缓冲液

PBS containing 0.09 % (W/V) Sodium Azide as preservative

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原

LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

别名

LC3B

背景

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.Synonyms: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B

基因ID

81631

NCBI登录号

NP_073729

途径

Autophagy