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PD-L1 抗体 (AA 24-153)

This anti-PD-L1 antibody is a 小鼠单克隆 antibody detecting PD-L1 in FACS 和 ELISA. Suitable for 人.

Western blot analysis using CD274 mAb against human CD274 (AA: 24-153) recombinant protein. (PD-L1 抗体 (AA 24-153))

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng) (PD-L1 抗体 (AA 24-153))

Western blot analysis using *** mAb against HEK293 (1) and *** (AA: ***)-hIgGFc transfected HEK293 (2) cell lysate. (PD-L1 抗体 (AA 24-153))

Flow cytometric analysis of Hela cells using CD274 mouse mAb (green) and negative control (red). (PD-L1 抗体 (AA 24-153))

4 images

产品编号 ABIN5542485

发货至: 中国

Datasheet as PDF

Quick Overview for PD-L1 抗体 (AA 24-153) (ABIN5542485)

抗原

See all PD-L1 抗体

PD-L1 (CD274 (PD-L1))

适用

273
142
72
6
6
5
5
1
1

人

宿主

153
145
20
5
4
4
2
1

小鼠

克隆类型

单克隆

标记

This PD-L1 antibody is un-conjugated

应用范围

194
145
95
73
45
45
44
40
28
15
11
10
7
7
5
3
3
2
2
1
1
1
1
1
1

Flow Cytometry (FACS), ELISA

克隆位点

7D2A10

Discover our top product PD-L1 Primary Antibody

抗原表位
72

29

16

15

14

11

11

10

7

7

7

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 24-153

原理

CD274 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human CD274 (AA: 24-153) expressed in E. Coli.

亚型

IgG2b
anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 19-238) antibody
PD-L1 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 9B5F4 unconjugated

anti-CD274 (PD-L1) (AA 39-191) antibody
PD-L1 适用: 人, 小鼠 WB, FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal PDL1-2746 unconjugated

anti-CD274 (PD-L1) (AA 24-153) antibody
PD-L1 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 6D9A2 unconjugated

anti-CD274 (PD-L1) (AA 48-290) antibody
PD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 34-241) antibody
PD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 19-238) antibody
PD-L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (C-Term) antibody
KO Validated PD-L1 适用: 人 WB, FACS, IF, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 201-290) antibody
PD-L1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-234) antibody
PD-L1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
抗原

PD-L1 (CD274 (PD-L1))

别名

CD274

背景

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.

分子量

33.3 kDa

基因ID

29126

UniProt

Q9NZQ7

途径

Cancer Immune Checkpoints

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