Transferrin 抗体 (N-Term)
Quick Overview for Transferrin 抗体 (N-Term) (ABIN5542240)
抗原
See all Transferrin (TF) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- This antibody recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
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交叉反应 （详细）
- Does not react with Bovine, Sheep, Canine (Dog), Equine (Horse).
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纯化方法
- Precipitation methods and ion exchange chromatography (> 95% pure by SDS-PAGE)
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免疫原
- Purified porcine transferrin
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亚型
- IgG1
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anti-Transferrin (TF) (AA 361-683) antibody
TF 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Pig WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 22-176) antibodyTF 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 551-650) antibodyTF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Transferrin (TF) (AA 284-575) antibodyTF 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Monoclonal 9B3 unconjugated
anti-Transferrin (TF) (AA 432-466) antibodyTF 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55980 unconjugated
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应用备注
- Western blot : non-reducing conditions. Immunohistochemistry on paraffin sections: 10 μg/mL. Positive control: placenta. Immunocytochemistry. ELISA. RIA. Functional application: blocks binding of transferrin to the receptor.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 95 % pure by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS), approx. pH 7.4 0.2 μm filter sterilized
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
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有效期
- 12 months
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- Transferrin (TF)
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别名
- transferrin
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背景
- Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 μmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH . Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
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UniProt
- P09571
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途径
- Transition Metal Ion Homeostasis
抗原
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