PARK7/DJ1 抗体 (AA 1-187)
Quick Overview for PARK7/DJ1 抗体 (AA 1-187) (ABIN5542007)
抗原
See all PARK7/DJ1 (PARK7) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-187
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特异性
- This antibody reacts with DJ-1.
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交叉反应 （详细）
- Does not react with Mouse (NIH/3T3, P19, BaF/3) and Rat (PC12).
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免疫原
- Recombinant full-length human DJ-1 (1-187 a.a.)
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亚型
- IgG1
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anti-Parkinson Protein 7 (PARK7) (AA 1-189) antibody
PARK7 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (C-Term) antibodyVerified PARK7 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (AA 101-189) antibodyPARK7 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (C-Term) antibodyPARK7 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (AA 2-189) antibodyPARK7 适用: 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (AA 1-188) antibodyPARK7 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (AA 1-189) antibodyPARK7 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (AA 2-189) antibodyPARK7 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (AA 1-189) antibodyPARK7 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 7 (PARK7) (AA 130-164), (C-Term) antibodyPARK7 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB49681 unconjugated
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应用备注
- Western blot: 1-10 μg/mL for chemiluminescence detection system.
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实验流程
- SDS-PAGE & Western Blotting 1) Wash the cells 3 times with PBS and suspend with 10 volumes of cold Lysis buffer (50 mM Tris-HCl, pH 7.2, 250 mM NaCl, 0.1 % NP-40, 2 mM EDTA, 10 % glycerol) containing appropriate protease inhibitors. Incubate it at 4 o C with rotating for 30 minutes, then sonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4 o C and transfer the supernatant to another tube. Measure the protein concentration of the supernatant and add the Lysis buffer to make an 8 mg/mL solution. 3) Mix the sample with an equal volume of Laemmli's sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μ L of the sample per lane in a 1 mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm 2 for 1 hour in a semi-dry transfer system. (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20 % MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10 % skimmed milk (in PBS, pH 7.2) for 1 hour at room temperature, or overnight at 4 °C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7.2 containing 1 % skimmed milk as suggested in the APPLICATIONS for 1 hour at room temperature. (The optimal antibody concentration will depend on the experimental conditions.) 8) Wash the membrane with PBS-T [0.05 % Tween-20 in PBS] (5 minutes x 6 times). 9) Incubate the membrane with the 1:10,000 HRP-conjugated anti-mouse IgG diluted with 1 % skimmed milk (in PBS, pH 7.2) for 1 hour at room temperature. 10) Wash the membrane with PBS-T (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriate chemiluminescence reagents for 1 minute. Remove extra reagent from the membrane by dabbing with a paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 1 minute. Develop the film as usual. The conditions for exposure and development may vary. (Positive controls for Western blotting Jurkat, Raji, HeLa)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Buffer System: Protein A agarose, PBS containing 50 % glycerol, pH 7.2. Contains no preservatives.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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- PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))
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别名
- dj-1,park7
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背景
- DJ-1 (PARK7/CAP1/RS) was originally cloned as a putative oncogene capable of transforming NIH/3T3 cells in cooperation with h-ras, a protein expressed in sperm, and a regulator of RNA-protein interactions. DJ-1 has also been isolated as a gene associated with autosomal ea rly-onset Parkinson's disease (PD). Taken together, DJ-1 appears to be involved in diverse biological processes. First, several lines of evidence suggest that DJ-1 plays a role in the oxidative stress response. In cultured mammalian cells, DJ-1 quenches reactive oxygen species and is converted into a variant with a more acidic isoelectric poin t. Therefore, DJ-1 protects against reactive oxygen specie s-induced cell death, and its suppression with small interfe ring RNA (siRNA) sensitizes cells to such insults. Second, DJ-1 modulates transcription through interaction with DJ-1-binding protein as well as with protein inhibitor of activated STAT (PIAS). The latter modulates the activity of various transcription factors. Third, DJ-1 has been recognized as a regulatory subunit of an RNA-binding protein. Fourth, DJ-1, which is structurally related to the molecular chaperone Hsp31, may have chaperone activity itsel f, preventing heat-induced aggregation of substrate proteins, including α -synuclein. In addition, several lines of evidence suggest that DJ-1 plays a role in human tumorigenesis. First, breast cancer patients have elevated levels of circulating DJ-1 and anti-DJ-1 autoantibodies compared to healthy and non-breast cancer patients. Secondly, DJ-1 protein is increased in primary non-small cell lung carcinoma sa mples. Thirdly, treatment of cells from the human lung cancer cell line NCI-H157 with paclitaxel and MEK inhibitor U0126 leads to a decrease in DJ-1 protein expression.
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UniProt
- Q99497
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport
抗原
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