BUB3 抗体 (AA 1-328)
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Quick Overview for BUB3 抗体 (AA 1-328) (ABIN5541713)
抗原
See all BUB3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-328
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纯化方法
- Protein-A affinity chromatography
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免疫原
- Recombinant human BUB3 (1-328aa) purified from E. coli.
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亚型
- IgG2b
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应用备注
- The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
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别名
- bub3
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背景
- BUB3 (budding uninhibited by benzimidazoles 3 homolog) is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments.
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UniProt
- O43684
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途径
- Maintenance of Protein Location
抗原
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