SIGLEC5 抗体 (FITC)
Quick Overview for SIGLEC5 抗体 (FITC) (ABIN5541400)
抗原
See all SIGLEC5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with mouse SiglecE.
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纯化方法
- Protein G agarose
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免疫原
- Mouse SiglecE transfected L cell
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亚型
- IgG2b
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anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-230) antibody
SIGLEC5 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3F5A3 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) antibodySIGLEC5 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibody (APC)SIGLEC5 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 1A5 APC
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 147-335) antibodySIGLEC5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (N-Term) antibodySIGLEC5 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 452-551) antibodySIGLEC5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 458-551) antibody (Biotin)SIGLEC5 适用: 人 WB 宿主: 兔 Polyclonal Biotin
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibodySIGLEC5 适用: 人, Non-Human Primate ELISA, IP, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal 1A5 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibody (PE)SIGLEC5 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 1A5 PE
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 18-135) antibodySIGLEC5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: 50 μg/mL (final concentration). For details see protocol below.
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实验流程
- Flow cytometric analysis for floating cells Protocol 1 We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2 % fetal calf serum (FCS) and 0.1 % NaN 3]. 2) Resuspend the cells with washing buffer (5x10 6 cells/mL). 3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 10 μ L of normal goat serum containing 1 mg/mL normal human IgG and 0.1 % NaN 3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature (20~25 o C). 5) Add 20 μL of the FITC labeled anti-mouse SiglecE monoclonal antibody (8D2) (50 μ g/mL) diluted with the washing buffer. Mix well and incubate for 30 minutes at room temperature (20~25 o C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 7) Add 20 μL of 1:50 PE conjugated anti-mouse CD11c (Pharmingen: code no.557401) diluted with the washing buffer. Mix well and incubate for 15 minutes at room temperature (20~25 o C). 8) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer. Positive control for flow cytometry mouse splenocytes Protocol 2 We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 2) Resuspend the cells with PBS containing 25 % normal goat serum, 1 mg/mL normal human IgG and 0.1 % NaN 3 (5x10 6 cells/mL). 3) Add 20 μ L of the FITC labeled anti-mouse SiglecE monoclonal antibody (8D2) (50 μ g/mL) diluted with the washing buffer into each tube. 4) Add 50 μ L of the cell suspension into each tube. Mix well and incubate for 30 minutes at room temperature (20~25 o C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 50 μ L of the washing buffer.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C. Shelf life: one year from despatch.
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有效期
- 12 months
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- SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
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别名
- cd170,siglec5
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背景
- SilecE (sialic acid-binding immunoglobulin-like lectin E/SigLec5/CD170) is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. SiglecE is expressed on neutrophil and monocyte popul ations, both in the blood and bone marrow, as a dimeric, disulfide linked, 140 kDa type I membrane protein containing cytoplasmic immune receptor-based tyrosi ne signaling motifs. SiglecE recruits the SH2-domain-containing protein tyrosine phosphatases SHP-1 and SHP-2, which block signal transduction through dephosphorylation of signaling molecules. Thus SiglecE acts as an inhibitory receptor for ligand induced tyrosine phosphorylation.
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UniProt
- Q920G3
抗原
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