NEFH 抗体
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北京 101111
Quick Overview for NEFH 抗体 (ABIN5540977)
抗原
See all NEFH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- RNF405 reacts exclusively with the phosphorylated isoform of the 200 kD Neurofilament protein.
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纯化方法
- Purified
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免疫原
- Neurofilament preparation of calf brain tissue.
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亚型
- IgG2a
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anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal NF421 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C12F3 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4F2G12 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 1-100) antibodyNEFH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig, Cow, 兔, 豚鼠, Gerbil, Cat WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal NE14 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 8H10 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 21-120) antibodyNEFH 适用: 人, 大鼠, 小鸡 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 821-1020) antibodyNEFH 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin biotinylated Horseradish Peroxidase complex (ABC) as detection reagent, and 1/100-1/500 for Immunoblotting applications.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Buffer System: PBS Preservatives: 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.
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- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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别名
- neurofilament h (200 kd)
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背景
- Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
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UniProt
- P12036
抗原
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